| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 81 | Thermotoga maritima | ACCTCACGTCGCCAATGT | TCCGGTTTTCGCACTGGT | 59.26 | 59.49 | 235 | 95 |
100.00%
|
100.00%
|
| Primer 82 | Thermotoga maritima | CAAGGAAGGTCACCGGTGT | CAGGCTTCTGAGGAACGCA | 59.55 | 60.00 | 242 | 94 |
100.00%
|
100.00%
|
| Primer 83 | Thermotoga maritima | GACGTGGATGTGAAAGCGC | TCTTCCTCCTCGCCTTCCA | 59.87 | 59.92 | 188 | 92 |
100.00%
|
100.00%
|
| Primer 84 | Thermotoga maritima | TTGGTTTTGCAGCTTCGGC | ACTTCGCGAGCGTGGATT | 59.93 | 59.74 | 255 | 90 |
100.00%
|
100.00%
|
| Primer 85 | Thermotoga maritima | CGTTGCCTTCGATGCGTTT | ACTTCGCGAGCGTGGATT | 59.79 | 59.74 | 233 | 90 |
100.00%
|
100.00%
|
| Primer 86 | Thermotoga maritima | TCGGCTACACCACAACGT | ACGGATCGTTCACGCTGA | 58.87 | 59.04 | 180 | 90 |
100.00%
|
100.00%
|
| Primer 87 | Thermotoga maritima | AGGGCTGGGAGATTCTCGT | TGAGCAACGTTGTCCGCA | 60.00 | 60.20 | 168 | 90 |
100.00%
|
100.00%
|
| Primer 88 | Thermotoga maritima | GTGTGAGGGACTGTGAGCTC | TTGCGAGCTTTCCGGTGA | 60.04 | 59.58 | 159 | 88 |
100.00%
|
100.00%
|
| Primer 89 | Thermotoga maritima | GAGTGTGAGGGACTGTGAGC | TTGCGAGCTTTCCGGTGA | 60.04 | 59.58 | 161 | 88 |
100.00%
|
100.00%
|
| Primer 90 | Thermotoga maritima | AGTGTGAGGGACTGTGAGC | TTGCGAGCTTTCCGGTGA | 58.95 | 59.58 | 160 | 88 |
100.00%
|
100.00%
|